This study investigated the impact of chemical inducers on the co-production of yeast oil (YO) and β-carotene from purified biodiesel-derived crude glycerol. The objective was to enhance YO and β-carotene co-production in Rhodosporidium toruloides through the application of individual and combined inducers at both flask and bioreactor scales. Among the individual inducers, 1 % w/v Tween 20 (TW) and 10 ppm ethephon (EP) significantly increased total yeast oil (TO) and total β-carotene (TC) concentrations, respectively. When TW and EP were used together, TO and TC production increased by 2.0 and 2.6-fold, respectively in the bioreactor compared to the flask. The YO primarily consisted of C16 and C18 long-chain fatty acids, and the β-carotene produced showed functional similarities to commercial β-carotene. This research highlights the potential of biodiesel waste as a sustainable feedstock for co-producing YO and β-carotene, with the dual-inducer strategy providing a simple and effective method for enhancing production efficiency.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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