July 09, 2013
SGD now provides links to LoQate (the localization and quantitation atlas of the yeast proteome) from the Protein Information section of the Locus Summary Pages. The LoQate database provides localization and abundance data for 5300 yeast proteins at single-cell resolution under three different stress conditions: DTT, H2O2, and nitrogen starvation (Breker et al, 2013, J Cell Biol. 200(6), 839-850). Thanks to Maya Schuldiner for helping us set up the links.
Categories: New Data, Website changes
May 28, 2013
YeastMine, SGD’s powerful search and retrieval tool, has been upgraded to use InterMine version 1.1 software. Highlights of this release include a new format for the template results page, the addition of PantherDB and Homologene homolog data, an improved representation of Gene Ontology (GO) information, the ability to set background population within the GO enrichment widget, and an option to share lists with other users. In addition to the existing video tutorials, a new Help document describes some common queries. See an overview of these new features in the video below, New, Fun YeastMine 1.1!:
New, Fun YeastMine 1.1! from yeastgenome on Vimeo.
Categories: Data updates, New Data
March 05, 2013
Sixty new datasets have been added to our expression analysis tool at SGD, facilitating the rapid identification of co-expressed genes based on patterns of expression shared with query gene(s) across the entire collection. Expression data are now available at SGD from a comprehensive collection of 430 datasets representing 9190 microarrays from a total of 286 publications. The expression analysis tool can be accessed via the Expression tab and Expression Summary histogram located on Locus Summary pages, or using the ‘Expression’ option in the Function pulldown in the menu bar at the top of SGD pages. The new data will by default be included with the previous data when using the ‘New Search’, ‘Show Expression Levels’, or ‘Dataset Listing’ options. Alternatively, the new datasets can be specifically filtered using the dataset tag ‘not yet curated’. All of the RNA expression data are available for download in expression directory. Datasets are grouped by publication and are in PCL format.
Categories: New Data
June 23, 2012
SGD now provides links from both the Locus Summary and Interactions pages for each S. cerevisiae ORF to DRYGIN (Data Repository of Yeast Genetic Interactions), a database of quantitative genetic interactions of S. cerevisiae (Koh et al., 2010). These genetic interactions were determined from SGA double-mutant arrays conducted in Charles Boone’s laboratory at the University of Toronto, and include both published data (Costanzo et al., 2010) and new interactions released by the Boone laboratory as they become available. Clicking on a DRYGIN link in SGD from an ORF’s Locus Summary or Interactions page goes directly to the DRYGIN search results page for that ORF, which lists both positive and negative genetic interactions as well as any genetic correlations for the given ORF.
Categories: New Data
Tags: DRYGIN, genetic interactions, SGA array
April 23, 2012
SGD has added a new mix of data tracks to our GBrowse genome viewer from seven publications covering transcriptome exploration via tiling microarrays (David et al. 2006), genomic occupancy of RNA polymerase II and III and associated factors (Kim et al. 2010; Ghavi-Helm 2008), 3′ end processing (Johnson et al. 2011), histone H2BK123 monoubiquitination (Schulze et al. 2011) and high-resolution ChIP by a novel method called ChIP-exo (Rhee et al. 2011; Rhee et al. 2012). Download data tracks, metadata and supplementary data by clicking on the ‘?’ icon on each data track within GBrowse or directly from the SGD downloads page. We welcome new data submissions pre- or post-publication and invite authors to work with us to integrate their data into our GBrowse and PBrowse viewers. Please contact us if you are interested in participating or have questions and comments. Happy browsing!
Categories: New Data
Tags: ChIP-exo, histone modifications, RNA polymerase II, RNA polymerase III, transcriptome
February 14, 2012
RNA expression data that are included in SGD’s SPELL expression analysis tool are now available for download in the expression directory. Datasets have been grouped by publication and are in PCL format.
LiftOver files that allow conversion of chromosomal coordinates between different S. cerevisiae genome versions are also now available for download via the genome_releases link in the sequence directory.
Categories: New Data, Website changes
January 26, 2012
SGD has added a mélange of data tracks to our GBrowse genome viewer from six publications covering various applications of high-throughput sequencing, including genome-wide distributions of DNase I-protected genomic footprints (Hesselberth et al. 2009), recombination-associated double strand breakpoints (Pan et al. 2011), polyadenylation sites (Ozsolak et al. 2010), antisense ncRNAs (Yassour et al. 2010), cryptic unstable transcripts (CUTs) (Neil et al. 2009) and Xrn1-sensitive unstable transcripts (XUTs) (van Dijk et al. 2011). You can now also easily download data tracks, metadata and supplementary data by clicking on the ‘?’ icon on each data track within GBrowse. Please watch our video tutorial for more information on how to download data from GBrowse. We welcome new data submissions pre- or post-publication and invite authors to work with us to integrate their data into our GBrowse and PBrowse viewers. Please contact us if you are interested in participating or have questions and comments. Happy browsing!
View Downloading GBrowse Data at SGD on Vimeo.
Categories: New Data, Tutorial
January 25, 2012
Links to YPL+ (the Yeast Protein LocalizationPlus Database) have been added to the “Protein Information” section of SGD Locus Summary pages. YPL+ is a recently upgraded version of the YPL image database, and has been expanded to include GFP-localization data for more than 3500 genes. Data in YPL+ are derived from a collection of GFP fusion constructs generated by C-terminal chromosomal tagging (Huh et al., 2003, Nature 425, 686-691) as well as a collection of proteins involved in lipid-metabolism, constructed by in vivo recombination (Natter et al., 2005, Mol. Cell. Proteomics 4(5), 662-672). Thanks to Sepp Kohlwein for help in setting up these links.
Categories: New Data, Website changes
August 05, 2011
We have added new data tracks to our GBrowse genome viewer from six publications covering various histone acetylation and methlyation modifications (Guillemette et al. 2011; Kirmizis et al. 2007; Liu et al. 2005 and Pokholok et al. 2005) and the mapping of histone variant H2A.Z (Albert et al. 2007 and Guillemette et al. 2005). We welcome new data submissions pre- or post-publication and invite authors to work with us to integrate their data into our GBrowse and PBrowse viewers. Please contact us if you are interested in participating or have questions and comments.
Categories: New Data
June 09, 2011
We’ve added new data tracks to our GBrowse genome viewer from eight publications, including recent surveys of nucleosome occupancy and positioning (Kaplan et al., 2009; Field et al., 2008; Mavrich et al., 2008; Lee et al., 2007); ncRNAs and RNA secondary structures (Lardenois et al., 2011 and Kertesz et al., 2010); and transcription factor and RNA Pol II occupancy (Venters et al., 2011 and Mayer et al., 2010). We have also added a data track to our Pbrowse proteome browser, displaying mature N-termini of mitochondrial proteins as determined by Vögtle et al., 2009. Both GBrowse and PBrowse have been upgraded to the most recent software with several new features.
We invite authors to work with us to integrate their data into our GBrowse and PBrowse viewers pre- and/or post-publication as we move forward. Watch for the regular addition of new tracks to SGD’s GBrowse and PBrowse in the future! Please contact us if you are interested in participating or have questions and comments.
Categories: Data updates, New Data